INTRACORONARY SUPEROXIDE DISMUTASE DURING THE INITIAL BURST OF FREE RADICALS

Superoxide dismutase Superoxide dismutase

Free radicals and inflammation. Protection of phagocytosine leukocytes by superoxide dismutase.

Isolated human polymorphonuclear leukocytes engaged in phagocytosis liberate superoxide radical and hydrogen peroxide into the surrounding medium. These two chemical species react to produce the hydroxyl radical, which attacks the leukocyte and leads to premature death of the cell. The hydroxyl radical may be scavenged by mannitol, or its formation can be prevented by the addition of superoxide...

Superoxide free radicals are produced in glyoxysomes.

The production of superoxide free radicals in pellet and supernatant fractions of glyoxysomes, specialized plant peroxisomes from watermelon (Citrullus vulgaris Schrad.) cotyledons, was investigated. Upon inhibition of the endogenous superoxide dismutase, xanthine, and hypoxanthine induced in glyoxysomal supernatants the generation of O(2) (-) radicals and this was inhibited by allopurinol. In ...

Mitochondrial complex I deficiency leads to increased production of superoxide radicals and induction of superoxide dismutase.

Mitochondria were isolated from skin fibroblast cultures derived from healthy individuals (controls) and from a group patients with complex I (NADH-CoQ reductase) deficiency of the mitochondrial respiratory chain. The complex I deficient patients included those with fatal infantile lactic acidosis (FILA), cardiomyopathy with cataracts (CC), hepatopathy with tubulopathy (HT), Leigh's disease (LD...

Evidence against the generation of free hydroxyl radicals from the interaction of copper,zinc-superoxide dismutase and hydrogen peroxide.

Prior spin trapping studies reported that H(2)O(2) is metabolized by copper,zinc-superoxide dismutase (SOD) to form (.)OH that is released from the enzyme, serving as a source of oxidative injury. Although this mechanism has been invoked in a number of diseases, controversy remains regarding whether the hydroxylation of spin traps by SOD is truly derived from free (.)OH or (.)OH scavenged off t...